Then a dichroic mount is assembled and installed to direct the laser beam into the objective lens of a standard inverted microscope.
The protocol begins with the assembly and alignment of beam-conditioning optics at the output of a femtosecond laser. Here, we present a step-by-step protocol for constructing a femtosecond laser microsurgery setup for use with a widely available compound fluorescence microscope. Steinmeyer, Joseph D Gilleland, Cody L Pardo-Martin, Carlos Angel, Matthew Rohde, Christopher B Scott, Mark A Yanik, Mehmet Fatihįemtosecond laser microsurgery is a powerful method for studying cellular function, neural circuits, neuronal injury and neuronal regeneration because of its capability to selectively ablate sub-micron targets in vitro and in vivo with minimal damage to the surrounding tissue. For a correct position of the place where the laser pulse strikes, we used an original system consisting of two red laser diodes mounted on each side of the binocular One of the advantages of this laser system is taht the output energies can be varied widely (0.8-15 mJ), making a great numbers of applications in clinical ophthalmology possible.Ĭonstruction of a femtosecond laser microsurgery system. In order to cover the medical domain of the energies, we calibrate eleven attenuation filters using ratiometric method. We had to design an optical scheme of the laser to settle the radiation route. This laser-stereomicroscope system is used for eye examination and for microsurgical proceedings like posterior capsulotomy and pupilar membranectomy. We design a specific ophthalmic system, containing a Q-switch Nd:YAG laser, an optical stereomicroscope and an aiming system.
The Nd:YAG solid state laser can be used in ophthalmologic microsurgery because of its specific wavelength of 1064 nm, which has the property to penetrate the transparent medium of the eye. Nd:YAG laser system for ophthalmic microsurgery
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